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Tscm cells from the blood and MLNs of patients with CD with treatment showed distinct IFN-γ expression levels. (A) Flow cytometric analysis of PBMCs and lymphocytes from patients with CD; Tscm cells are defined as CD3 + CD4 + CD45RA + <t>/CD45RO</t> − CCR7 + CD62L + CD95 + CD122 + or CD3 + CD8 + /CD4 − CD45RA + /CD45RO − CCR7 + CD62L + CD95 + CD122 + cells. The frequencies of CD4 + Tscm and CD8 + Tscm cells in PBMCs and healthy MLNs and the lesions of MLNs from patients with CD with treatment. (B) Representative flow cytometric analysis of IFN-γ-, IL-17-, and TNF-α-producing CD4 + Tscm and CD8 + Tscm cells in PBMCs and MLNs from patients with CD and healthy donors. (C) The mean frequencies (± SEM) of IFN-γ-, IL-17-, and TNF-α-expressing CD4 + Tscm and CD8 + Tscm cells in the blood and MLNs. *, P<0.05; unpaired Student t -test. (D) Plot showing the frequencies of IFN-γ-, IL-17-, and TNF-α-secreting CD4 + Tscm and CD8 + Tscm cells in blood and MLNs from patients with CD and healthy donors expressed as the mean ± SEM. *, P<0.05; unpaired Student t -test. Tscm, stem cell-like memory T; HD-PBMCs, PBMCs from healthy donors; PBMCs, peripheral blood mononuclear cells; CD-PBMCs, PBMCs from patients with CD; CD, Crohn’s disease; N-M-Lys, healthy MLNs from patients with CD; MLNs, mesenteric lymph nodes; CD-M-Lys, lesions of the MLNs from patients with CD; SSC, side scatter; IFN-γ, interferon-γ; IL, interleukin; TNF-α, tumor necrosis factor-α; SEM, standard error of the mean.
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Image Search Results


Journal: iScience

Article Title: Migration of human T cells can be differentially directed by electric fields depending on the extracellular microenvironment

doi: 10.1016/j.isci.2024.109746

Figure Lengend Snippet:

Article Snippet: Anti-human CD45RO mouse IgG2a - FITC , ImmunoTools , Cat#: 21336453 Lot: 277066.

Techniques: Recombinant, Cell Isolation, Isolation, Software

Tscm cells from the blood and MLNs of patients with CD with treatment showed distinct IFN-γ expression levels. (A) Flow cytometric analysis of PBMCs and lymphocytes from patients with CD; Tscm cells are defined as CD3 + CD4 + CD45RA + /CD45RO − CCR7 + CD62L + CD95 + CD122 + or CD3 + CD8 + /CD4 − CD45RA + /CD45RO − CCR7 + CD62L + CD95 + CD122 + cells. The frequencies of CD4 + Tscm and CD8 + Tscm cells in PBMCs and healthy MLNs and the lesions of MLNs from patients with CD with treatment. (B) Representative flow cytometric analysis of IFN-γ-, IL-17-, and TNF-α-producing CD4 + Tscm and CD8 + Tscm cells in PBMCs and MLNs from patients with CD and healthy donors. (C) The mean frequencies (± SEM) of IFN-γ-, IL-17-, and TNF-α-expressing CD4 + Tscm and CD8 + Tscm cells in the blood and MLNs. *, P<0.05; unpaired Student t -test. (D) Plot showing the frequencies of IFN-γ-, IL-17-, and TNF-α-secreting CD4 + Tscm and CD8 + Tscm cells in blood and MLNs from patients with CD and healthy donors expressed as the mean ± SEM. *, P<0.05; unpaired Student t -test. Tscm, stem cell-like memory T; HD-PBMCs, PBMCs from healthy donors; PBMCs, peripheral blood mononuclear cells; CD-PBMCs, PBMCs from patients with CD; CD, Crohn’s disease; N-M-Lys, healthy MLNs from patients with CD; MLNs, mesenteric lymph nodes; CD-M-Lys, lesions of the MLNs from patients with CD; SSC, side scatter; IFN-γ, interferon-γ; IL, interleukin; TNF-α, tumor necrosis factor-α; SEM, standard error of the mean.

Journal: Translational Gastroenterology and Hepatology

Article Title: Crohn’s disease treatment and memory T-cell subset changes: insights from a case series

doi: 10.21037/tgh-23-21

Figure Lengend Snippet: Tscm cells from the blood and MLNs of patients with CD with treatment showed distinct IFN-γ expression levels. (A) Flow cytometric analysis of PBMCs and lymphocytes from patients with CD; Tscm cells are defined as CD3 + CD4 + CD45RA + /CD45RO − CCR7 + CD62L + CD95 + CD122 + or CD3 + CD8 + /CD4 − CD45RA + /CD45RO − CCR7 + CD62L + CD95 + CD122 + cells. The frequencies of CD4 + Tscm and CD8 + Tscm cells in PBMCs and healthy MLNs and the lesions of MLNs from patients with CD with treatment. (B) Representative flow cytometric analysis of IFN-γ-, IL-17-, and TNF-α-producing CD4 + Tscm and CD8 + Tscm cells in PBMCs and MLNs from patients with CD and healthy donors. (C) The mean frequencies (± SEM) of IFN-γ-, IL-17-, and TNF-α-expressing CD4 + Tscm and CD8 + Tscm cells in the blood and MLNs. *, P<0.05; unpaired Student t -test. (D) Plot showing the frequencies of IFN-γ-, IL-17-, and TNF-α-secreting CD4 + Tscm and CD8 + Tscm cells in blood and MLNs from patients with CD and healthy donors expressed as the mean ± SEM. *, P<0.05; unpaired Student t -test. Tscm, stem cell-like memory T; HD-PBMCs, PBMCs from healthy donors; PBMCs, peripheral blood mononuclear cells; CD-PBMCs, PBMCs from patients with CD; CD, Crohn’s disease; N-M-Lys, healthy MLNs from patients with CD; MLNs, mesenteric lymph nodes; CD-M-Lys, lesions of the MLNs from patients with CD; SSC, side scatter; IFN-γ, interferon-γ; IL, interleukin; TNF-α, tumor necrosis factor-α; SEM, standard error of the mean.

Article Snippet: The following panel of mouse anti-human monoclonal antibodies (mAbs) used were all purchased from BD Biosciences (Franklin Lakes, NJ, USA) or eBioscience (San Diego, CA, USA): anti-human CD3-APC.Cy7 (cat. 557832, SK7; BD Biosciences), anti-human CD4-Percp.Cy5.5 (cat. 560650, RPA-T4; BD Biosciences), anti-human CD8-Alexa Fluor 700 (cat. 56-0088-42, RPA-T8; eBioscience), anti-human CCR7-PE.eFluor610 (cat. 61-1979-42, 3D12; eBioscience), anti-human CD62L-APC (cat. 17-0629-42, DREG56; eBioscience), anti-human CD45RO-FITC (11-0457-42, UCHL1; eBioscience), anti-human CD95-PE.Cy7 (cat. 561633, DX2; BD Biosciences), anti-human CD103-APC (cat. 17-1037-41, Ber-ACT8; eBioscience), and anti-human CD122-PE (cat. 554525; BD Biosciences).

Techniques: Expressing

The frequencies of Trm-cell subsets in lymph nodes from patients with CD with treatment. (A) Flow cytometric analysis of CD69 and/or CD103 expression on CD3 + CD4 + Trm and CD3 + CD8 + Trm cells from patients with CD; Trm cells and their subsets are defined as CD3 + CD4 + CD45RO + CD69 + CD103 + , CD3 + CD8 + CD45RO + CD69 + CD103 + , CD3 + CD4 + CD45RO + CD69 + , CD3 + CD8 + CD45RO + CD69 + , CD3 + CD4 + CD45RO + CD103 + , or CD3 + CD8 + CD45RO + CD103 + cells. (B) The frequencies of CD4 + CD69 + CD103 + Trm and CD8 + CD69 + CD103 + Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. **, P<0.01; ***, P<0.001; unpaired Student t -test. (C) The proportions of CD4 + CD69 + CD103 − Trm and CD8 + CD69 + CD103 − Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. *, P<0.05; unpaired Student t -test. (D) The fractions of CD4 + CD103 + CD69 − Trm and CD8 + CD103 + CD69 − Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. *, P<0.05; **, P<0.01; unpaired Student t -test. Trm, tissue-resident memory T; N-SIM, healthy small intestinal mucosa from patients with CD; CD, Crohn’s disease; CD-SIM, lesions from the small intestinal mucosa from patients with CD; N-M-Lys, healthy MLNs from patients with CD; MLNs, mesenteric lymph nodes; CD-M-Lys, lesions of the MLNs from patients with CD.

Journal: Translational Gastroenterology and Hepatology

Article Title: Crohn’s disease treatment and memory T-cell subset changes: insights from a case series

doi: 10.21037/tgh-23-21

Figure Lengend Snippet: The frequencies of Trm-cell subsets in lymph nodes from patients with CD with treatment. (A) Flow cytometric analysis of CD69 and/or CD103 expression on CD3 + CD4 + Trm and CD3 + CD8 + Trm cells from patients with CD; Trm cells and their subsets are defined as CD3 + CD4 + CD45RO + CD69 + CD103 + , CD3 + CD8 + CD45RO + CD69 + CD103 + , CD3 + CD4 + CD45RO + CD69 + , CD3 + CD8 + CD45RO + CD69 + , CD3 + CD4 + CD45RO + CD103 + , or CD3 + CD8 + CD45RO + CD103 + cells. (B) The frequencies of CD4 + CD69 + CD103 + Trm and CD8 + CD69 + CD103 + Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. **, P<0.01; ***, P<0.001; unpaired Student t -test. (C) The proportions of CD4 + CD69 + CD103 − Trm and CD8 + CD69 + CD103 − Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. *, P<0.05; unpaired Student t -test. (D) The fractions of CD4 + CD103 + CD69 − Trm and CD8 + CD103 + CD69 − Trm cells in the small intestinal mucosa and MLNs from patients with CD with treatment. *, P<0.05; **, P<0.01; unpaired Student t -test. Trm, tissue-resident memory T; N-SIM, healthy small intestinal mucosa from patients with CD; CD, Crohn’s disease; CD-SIM, lesions from the small intestinal mucosa from patients with CD; N-M-Lys, healthy MLNs from patients with CD; MLNs, mesenteric lymph nodes; CD-M-Lys, lesions of the MLNs from patients with CD.

Article Snippet: The following panel of mouse anti-human monoclonal antibodies (mAbs) used were all purchased from BD Biosciences (Franklin Lakes, NJ, USA) or eBioscience (San Diego, CA, USA): anti-human CD3-APC.Cy7 (cat. 557832, SK7; BD Biosciences), anti-human CD4-Percp.Cy5.5 (cat. 560650, RPA-T4; BD Biosciences), anti-human CD8-Alexa Fluor 700 (cat. 56-0088-42, RPA-T8; eBioscience), anti-human CCR7-PE.eFluor610 (cat. 61-1979-42, 3D12; eBioscience), anti-human CD62L-APC (cat. 17-0629-42, DREG56; eBioscience), anti-human CD45RO-FITC (11-0457-42, UCHL1; eBioscience), anti-human CD95-PE.Cy7 (cat. 561633, DX2; BD Biosciences), anti-human CD103-APC (cat. 17-1037-41, Ber-ACT8; eBioscience), and anti-human CD122-PE (cat. 554525; BD Biosciences).

Techniques: Expressing